Regulation of Human Eosinophil Viability , Density , and Function by Granulocyte / Macrophage Colony - Stimulating Factor in the Presence of 3 T 3 Fibroblasts by William
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چکیده
Eosinophils, cells that are associated with asthma (1, 2), helminthic infections (3), and other clinical disorders, are thought to reside in the peripheral blood for <12 h and in connective tissues for several days (4). The in vitro survival of human peripheral blood eosinophils is limited to <2 d. Because the eosinophilia that normally accompanies helminthic infections is suppressed in athymic mice (5) and in normal rats or mice that have been depleted of their T lymphocytes (6-10), it has been concluded that eosinophilopoiesis is modulated by T cell factors. Granulocyte/macrophage colony-stimulating factor (GM-CSF)' is thought to be one of the T cell-derived factors that regulates eosinophils in vivo. Upon intravenous infusion in primates, GM-CSF is known to induce granulocy-tosis with eosinophilia (11), .and will induce human bone marrow progenitors to form colonies of eosinophils in vitro (12). Mature peripheral blood eosinophils may possess high-affinity GM-CSF receptors (13), and recent studies (14) have demonstrated that the short-term in vitro exposure of human eosinophils to biosynthetic (recombinant) human GM-CSF (rH GM-CSF) augments their anti-body-dependent killing of Schistosoma mansoni larvae and their calcium ionophore A23187-stimulated generation of leukotriene C4 (LTC 4). Hypodense eosinophils, defined by their lower-than-normal densities in discontinuous metrizamide or Percoll gradients, have been isolated from patients with idiopathic hypereosino-philic syndromes or from hosts chronically infected with S. mansoni. These less
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Conjunctival fibroblasts enhance the survival and functional activity of peripheral blood eosinophils in vitro.
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تاریخ انتشار 1987